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Its claimed by some coffee enema advocates that, before the advent of modern painkillers, Florence Nightingale used coffee enemas as a form of pain management in soldiers during the Crimean War, and doctors used them in the second world war

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J Cell Mol Med 8:20112, PMID: 15256068 Aracena-Parks P, Goonasekera SA, Gilman C, Dirksen RT, Hidalgo C, Hamilton SL (2006) Identification of cysteines involved in S -nitrosylation, S -glutathionylation, and oxidation to disulfides in RyR1