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Cells were resuspended in 30 mL of sterile water and spun down again at 3000 x g , at 4 C for 5 min, and the supernatant was discarded

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Hold the needle in place for about 5 to 10 seconds to allow the full dose to be delivered

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The short version: KPV has a mechanistically interesting anti-inflammatory profile through melanocortin receptor agonism and direct NF-B suppression

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