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Construction and transfection of overexpression plasmids To investigate the overexpression of ILF3 and PD-L1 in GC cells, the genes HOMO-ILF3-C-3xFLAG and HOMO-PD-L1-C-3xFLAG were cloned and inserted into the pcDNA3.1 vector to produce the pcDNA3.1-ILF3 and pcDNA3.1-PD-L1 plasmids

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Common qualifying conditions include type 2 diabetes, prediabetes, hypertension, dyslipidemia, obstructive sleep apnea, and a history of cardiovascular events

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