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Full centrifugation at low temperature (15 min at 12,000 g) to obtain supernatant, and the protein concentration was quantified by BCA kit (Epizyme, Shanghai, China), proteins electrophoresis using the 10% sodium dodecyl sulfate-polyacrylamide gel and transferred onto 0.45 m PVDF membranes (Millipore, United States)

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In our case, the tumor only presented a focally high Ki-67 index of 18%

Glutathione stands out for its role in detoxification, immune support, and cellular health

No significant difference was observed between the two tracings, and the area of the peak eluting at 4.29 min r.t

"Preclinical Pharmacology of Solriamfetol: Potential Mechanisms for Wake Promotion"