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Growth of methionine synthesis mutants in laboratory cultures The L-methionine synthesis mutants were first grown in 7H9-Tween-ADC liquid medium, both with and without B12 (AdoCbl, 10 g/mL), to assess their growth in the presence and absence of the vitamin and thus validate their phenotypes

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In contrast to the reversibly hyperoxidized cysteine sulfenylated proteins, irreversibly hyperoxidized sulfonylated cysteine is generally thought to be an impaired dead end product (e.g., inactivated protein) formed through protein aggregation 25