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Detection of deposited TCC (C5bC9) was done using a biotinylated mouse monoclonal antibody specific for a C9 neoepitope exposed upon C5b binding (Diatec Monoclonals, DIA 011-01) (diluted 1:5000 in PBS/T/S) together with AP-conjugated streptavidin (diluted 1:5000 in PBS/T/S) (Cytiva Life Sciences) and visualized by addition of AP substrate (Sigma-Aldrich)

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The protective effect of the viral oncoprotein was evident and statistically significant for the HaCaT cells, as described [29], [30], and for GSTP1-expressing MCF-7 cells, but not for the GSTP1-deficient MCF-7 cells (Figure 7A)

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And our process takes around 35 to 40 days according to the quantity which is ordered by our client