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Cells were incubated in click-reaction cocktail (2 mM copper sulfate, 10 M biotin-azide and 100 mM sodium ascorbate in PBS) for 1 h at 37 C and further incubated with Duolink Blocking Solution for 1 h at room temperature

Subramanian S, et al

After the first puncture, bacteriostatic water remains viable for research use for up to 28 days when stored correctly (refrigerated at 28C)

Acta Medica Iranica (2006) 44(3):15966

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