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The cells were then washed with PBS, and GSH and GSSG levels were determined by applying the GSH/GSSG-Glo assay according to manufacturer instructions

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In addition, the degradation of intracellular GPX4 through CMA pathways can lead to the accumulation of intracellular phospholipid peroxide and ferroptosis, and host cells usually use GPX4 for peroxide phospholipids reduction, thus protecting cells from death

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