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After incubation, cells were lysed using the Promega caspase 3/7 Glo kit and the intensity ofcaspase3/7 were read using Perkin-Elmer Enspire plate reader

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BPC-157 is a synthetic 15-amino acid peptide derived from a protein found in gastric juice

Lipo-toxicity occurs when adipose tissue accumulates in non-fat organs (e.g., liver, pancreas, and muscle tissue), dysfunctionalizing organelles, releasing excess ROS, leading to systemic inflammation and IR [116]

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