There are two phases of growth: initially horizontal (mainly involving the epithelium and lamina propria) and in more advanced stages, vertical, with invasion of corpus spongiosum and cavernosum
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Inconsistent signals across plates are most commonly caused by temperature differences between the plate and reagent at the time of addition (ensure both are equilibrated to room temperature for 30 minutes before mixing), insufficient cell lysis (increase mixing time or speed), or signal read outside the stable window (read plates within 10 minutes to 1 hour after stabilization)
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The primer sequences used were: forward primer Nlrp6 5-CTGGCGTCATTGTGGAACCTCT-3 and the reverse primer Nlrp6 5-TCTCACTCAGCTCCACAGAGGT-3
While the human GGT gene is not fully characterized, evidence suggests its existence in multiple copies within the human genome (36)
