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Briefly, GSH-depleted cells treated with ASA with or without NAC were trypsinized, washed in PBS and resuspended (110 6 cells/ml) in binding buffer (10 mM HEPES, pH 7.4, 140 mM NaCl, 2.5 mM CaCl 2 )

Bierl C, Voetsch B, Jin RC, Handy DE, Loscalzo J (2004) Determinants of human plasma glutathione peroxidase (GPx-3) expression

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Therefore, they wont be able to absorb the nutrients to benefit their health