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A new LC-MS/MS method for the clinical determination of reduced and oxidized glutathione from whole blood

Journal of Immunology

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After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C

This suggests that glutathione can indeed help fade dark spots