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For the GSH estimation, 100 L of the supernatant was mixed with 900 L of reaction buffer (0.1 M phosphate buffer, pH 7.5, containing 5 mM EDTA), and the absorbance was measured at 412 nm using a spectrophotometer as the blank value 20

By dividing the total radiation dose into multiple small fractions for treatment, not only can the therapeutic effect on the tumor be maintained, but normal tissues are also given more time for repair and recovery, thereby reducing the risk of long-term neurotoxicity [86, 87]

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