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Increasing amounts of NADPH (1-250 M) were subsequently added to the wells and the solutions imaged on a LSM880 confocal laser scanning microscope (Carl Zeiss AG, Oberkochen, Germany) with a 63x immersion objective (LD LCI Plan-Apochromat 63x/1.2 Imm Corr DIC M27) using a 440 nm pulsed excitation laser (LDH-D-C-440, 40 MHz repetition rate) managed by a PicoQuant FLIM module (Sepia PDL828-S, PMA Hybrid 40, MultiHarp 150 4N, Time Harp 260 PICO Dual) (PicoQuant, Berlin, Germany)
The practice is based on the belief that higher levels of Vitamin C can yield increased health benefits, particularly in terms of immune system support
Unlike regular sterile water, which requires single-dose use and immediate discarding after opening, bacteriostatic water can safely remain in a vial for extended periods
It helps regulate metabolism, muscle mass, energy levels, and fat distribution
Patients with primary hyperoxaluria type 2 have significant morbidity and require careful follow-up
