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2.5 Quantification of oxidative phosphorylation and glycolysis The Seahorse XF96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA, United States) was utilized to measure real-time integrated cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) as previously outlined ( 2.6 Western blotting (WB), real-time quantitative PCR (RT-qPCR) and reactive oxygen species (ROS) analysis WB analysis, RT-qPCR, and ROS analysis were performed as previously described (Supplementary Table S1
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This is partly because the drugs effects occur so fast, and the number of mechanisms that too much of the drug in the system can disable
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A meta-analysis of microRNA networks regulated by melatonin in cancer: Portrait of potential candidates for breast cancer treatment
