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The partially purified proteins in 10 kDa spin columns were desalted by washing twice with ice-cold buffer (25 mM MES [pH 6.0], 150 mM NaCl, 5% glycerol, 0.4% (w/v) n-Nonyl--d-glucopyranoside) and concentrated to about 10 mg ml 1 followed by loading onto a Superdex-200 gel filtration column (GE Healthcare Life Sciences, PA) to remove the contaminated proteins

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