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Insulin resistance, de novo lipogenesis, and dysregulated blood glucose are key hallmarks in the progression of NAFLD as part of the multiple-hit hypothesis (39)

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All participating dogs had previously been recruited based on specified criteria into a 12-month prospective, double-blinded, placebo-controlled randomized clinical trial in which dogs received rapamycin or placebo three times per week for six months

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It is a mixed ANOVA as there is a mixture of between groups and repeated measures variables