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A faint blue tint in the reconstituted GHK-Cu solution is characteristic of the copper(II) complex and directly indicates that the peptide is properly chelated with copper

Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP

This cycle allows one molecule of glutathione to protect the cell multiple times

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It has been extensively studied for its regenerative effects, particularly in healing soft tissue, reducing inflammation, and improving gut health