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Redness, itching, and localized swelling can occur with either medication, and these reactions are more common when the injection reaches muscle tissue or when technique is inconsistent

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coli BL21 (DE3) cells that were grown in LB medium at 37 C to an OD600 of 0.70.8 before being induced with 0.1 mM IPTG at 18 C for 16 h