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After freeze-thaw processing, supernatants were collected and stored at -80C until required ( 2.2 Cell viability assay Cell Counting Kit-8 (CCK-8) (Beyotime Biotech, China) was used to measure the corresponding optical density ratio marker cell viability levels in 96-well plates according to the manufacturers instructions

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Invitrogen) followed by injection of inactive Exendin-4 (E9)-Cy3 (50l, 0.5-2nmol in HBSS) via the tail vein

Elderly patients tend to have more fragile veins, which raises the chance of infiltration and bruising, while their slower metabolism affects how fast the body processes fluids

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