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Immunoblotting Protein samples in 1x NuPage LDS sample buffer, 25 mM DTT (or 50 mM TCEP pH 7.0) were loaded on to SDS PAGE gels (Invitrogen, XP04205) and transferred to nitrocellulose membranes

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Le glutathion rduit, ou GSH, est la seule forme active qui permet de dtoxifier et de jouer un vritable rle dantioxydant