GenJet In Vitro DNA Transfection Reagent and buffer were purchased from Tebu-bio (Heerhugowaard, Netherlands)
& Schramm, V
Most are peptides, but some are not
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The V3-V4 hypervariable regions of the 16S rRNA gene were amplified using primers 341 F and 785R, which included Illumina adapter sequences, following the 16S Metagenomic Sequencing Library Preparation protocol (Illumina, San Diego, CA, USA)