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Theranostics 11 , 614648 (2021)

After day 20 neurons were fed weekly, by replacing 1/10 volume of the old media with fresh growth media containing 2 M Ara-C

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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions