Upon thawing, it may not re-dissolve evenly, creating an unstable solution that can damage peptides
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Nitrous oxide can also deplete B12 levels
The NIH Office of Dietary Supplements, citing Institute of Medicine recommendations, advises that it is advisable for those older than 50 years to meet their RDA mainly by consuming foods fortified with vitamin B12 or a supplement containing vitamin B12. This recommendation reflects the fact that 10 to 30% of older adults lose the ability to efficiently absorb naturally occurring B12 from food
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Eating B12-rich foods like meat, eggs, and dairy or taking supplements daily can also help raise levels over time