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The authors first placed tetracycline response element (TRE) sequences next to the target DNA sequence, as TRE sequences have high affinity for the tetracycline-controlled transcriptional activator (tTA) protein
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Cells with impaired PERK-eIF2a-ATF4 signal transduction are more sensitive to hypoxic stress in vitro , indicating that the PERK-eIF2a-ATF4 pathway provides a survival advantage for cells under hypoxic conditions (75, 76), which is crucial for resistance to intracellular hypoxia, metabolic stress, and starvation
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