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In brief, slices washed with PBS three times, fixed with 4% paraformaldehyde for 30 min, incubated in 0.5% Triton X-100 for 5 min, then incubated in TUNEL reaction mixture for 60 min at 37C

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(8) There are several neurotransmitters and functions considered by scientists to be important, such as dopamine, nitrous oxide, prostaglandin, and other neuron systems