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Brain tissues were cut with a freezing microtome (Microm GmbH, Walldorf, Germany) into 30-m-thick coronal sections which were serially collected in 96-well plates containing a solution composed of 30% glycerol, 30% ethylene glycol, and 0.02% sodium azide in 0.1 M PBS, and then processed for immunohistochemistry or stored at -20 C

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The peptide does not affect liver metabolism in ways that would interact with alcohol processing