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The higher dose produced results comparable to tirzepatide, suggesting that dose intensity is a significant variable in GLP-1 agonist efficacy

The N-terminal tripeptide deletion in IGF-1 DES dramatically reduces its binding affinity for all IGFBPs by approximately 100-fold or more, while preserving or even slightly enhancing its affinity for the IGF-1 receptor itself

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To allow for equilibration of the system, the intestine/colon was perfused for 25 min before initiation of the experimental protocol

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