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Copper toxicity is associated with excessive oral ingestion rather than topical application, and absorption through intact skin at cosmetic doses is considered negligible

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Others, such as those seeking a more even tone, need several months for the benefits to develop

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After harvesting by centrifugation, cells were lysed in a buffer containing Tris-HCl, EDTA, DTT, and lysozyme, then sonicated and centrifuged

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