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Optional: If the lysate is too viscous, add RNase A (10 g/mL) and DNase I (5 g/mL) and incubate on ice for 10-15 minutes

Molecular biology of the androgen receptor

Doses of 2 to 4 times the therapeutic dose have only small effects, but the effect increases rapidly with doses above 8 times the normal therapeutic dose if P-450 activity is elevated