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Mice fasted for 24 h exhibit small intestinal atrophy, a decrease in intestinal weight, a decrease in crypt-villus height, and an increase in villus apoptosis (Shin et al., 2005)

They were then washed twice using F-12 with 0.1% BSA or 0.1% casein, 33 mM HEPES, and incubated for 2 h at 37 C

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It is strongly recommended to consider an UCD at any age in the presence of an acute or intermittent neurological deterioration or a psychiatric pathology, an acute hepatic failure or intoxication