2.8 CCK-8 assay HUVECs were evenly seeded into 96-well plates at a density of 1 10^4 cells per well, with a culture medium volume of 100 L per well
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Following the 48 h incubation, plates were centrifuged at 4500 x g for 15 mins
10.1016/S0896-6273(00)80837-5 59 KhodorovaA.NicolG
B12 is given as an intramuscular injection , usually into your upper arm or buttock