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The eluted proteins were dialyzed against buffer A using an Amicon Ultra-15 device with a molecular cutoff of 10 kDa (Millipore, UFC901008) and concentrated to 0.5 ml

This matters because animal results frequently fail to replicate in humans

DeCastroMNankovaBBShahPPatelPMallyPVMishraRet al

Mol Cell Biol 24 : 94149423

Reduction in Pigmentation Moreover, it effectively reduces dark spots, melasma, sun tan, acne marks, and uneven skin tone