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Total saliva was collected by fluid aspiration for 10 min using a capillary tube

PLoS One 12 (4), e0175499

Receptor internalisation was calculated from the loss of fluorescence intensity at the plasma membrane after 10 minutes of exendin-4 stimulation compared to baseline intensity for cells, or from the loss of fluorescence intensity after 5 minutes of exendin-4 stimulation versus vehicle for islets

If used in the wrong place in the stomach, this can lead to considerable complications or, because the stomach is such a large organ, have no effect at all

However, its crucial to approach this with realistic expectations and a focus on overall wellness

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