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In addition, the EGFP expression level determined by RT-qPCR also confirmed the enrichment efficiency of the cell sorting operation, with five times for pcDNA3.1-GST DAF -ATP6AP1L and 3.3 times for pcDNA3.1-GST CEAM7 -ATP6AP1L (Figure 6C)

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[DOI] [PubMed] [Google Scholar] 41.Miranda K.M., Paolocci N., Katori T., Thomas D.D., Ford E., Bartberger M.D., Espey M.G., Kass D.A., Feelisch M., Fukuto J.M., et al

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These approaches require solid foundational knowledge and careful attention to response