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The human CBS and L2HGDH gene cDNAs were synthesized with Twist Bioscience (South San Francisco, CA), with the sgRNA targeting regions replaced with wobble mutations

A critical paradox emerged: upregulated antioxidants (Gpx3, Mgst1) could not reverse GSH depletion, signaling exhausted compensation

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Integrating single-cell transcriptomic data across different conditions, technologies, and species