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For biotransformations, this can be overcome by cell permeabilization, for example, with polyethylenimine (Cnovas et al., 2005

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Decreased GSH then results in lipid peroxide accumulation and induction of ferroptosis

Although the following protocol has been used successfully to denature and re-nature GST fusion proteins, each fusion protein construct is unique and the exact denaturation and re-naturation conditions must be determined empirically