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In 2016, Jung et al

The glutathione biosynthetic pathway of Plasmodium is essential for mosquito transmission

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The protecting role of Ganoderma lucidum polysaccharides on the retinal neurovascular units in rats with retinal ischemia-reperfusion injury

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Over multiple cycles, this contributes to: Physical aggregation (clumping) of peptide molecules Potential denaturation of three-dimensional structure Gradual loss of biological activity in research assays The practical solution is simple: aliquot before freezing