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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates
J Mol Cell Cardiol 45:650660 Wahllander A, Soboll S, Sies H, Linke I, Mller M (1979) Hepatic mitochondrial and cytosolic glutathione content and the subcellular distribution of GSH-S-transferases
Neurochem Int, 2000;36: 97-112
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NaBPC157 (administered either in the ng or g/kg range, i.p.) significantly reduced the reactions in the writhing (inflammatory and non-inflammatory, prostaglandin-dependent and independent) and tail pinching tests
