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Cell lines, culture conditions, transfection and infection The Human SimpsonGolabiBehmel syndrome (SGBS) cell line was kindly supplied by Martin Wabitsch (University of Ulm, Germany) 45 and authenticated as preadipocytes based on their response to adipogenic cues (cocktails) and the expression of adipogenic marker genes

Coulomb interactions were calculated using the particle-mesh Ewald (PME) algorithm

Insulin (INS) and semaglutide (Sema) are first-line and commonly used medications for type 1 diabetes and type 2 diabetes, respectively

Apollo-NADP(+): a spectrally tunable family of genetically encoded sensors for NADP(+)