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However, pulling the acid end of the lipid tail to the opposite leaflet imposed a 7.5 kcal mol 1 free energy barrier due to the Glu-2xOEG linker being pulled past the phosphate headgroups

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The strength of the promoter in transgene therapy or modulation of the CRISPRa transcriptional activators will play a key role in determining the appropriate treatment and avoiding unpredictable or undesired treatment consequences