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Then, cDNA was prepared using high-capacity RNA- to-cDNA master mix (Applied Biosystems, 4368814) according to kit instructions

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SF-1 (NR5A1) expression is stimulated by the PKA pathway and is essential for the PKA-induced activation of LIPE expression in Y-1 cells

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With more research and better understanding, the peptide rules might change to be safe, helpful, and accessible to those who can benefit from them