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The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)

In rodent studies, BPC-157 administered intraperitoneally or subcutaneously shows rapid absorption and clearance, with detectable plasma levels dropping significantly within the first few hours

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Sci Rep 15 , 27257 (2025)