This was a remarkable conclusion, since the actual elements of protein secondary structure were introduced in 1951 by Linus Carl Pauling (19011994) and Robert Corey (18971971), who predicted the -helix and -sheet based on the hydrogen bonding of the protein backbone (Pauling and Corey, 1951), whereas the concept of secondary structure itself was formalized by Kaj Ulrik Linderstrm-Lang (18961959) in 1952 (Linderstrm-Lang, 1952)
However, preservative systems are only one component of contamination control
Some online medical supply stores sell it, but regulations and availability vary by region
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Each freeze-thaw cycle creates ice crystals that can physically disrupt peptide structure, and the process concentrates the peptide at the boundary between frozen and unfrozen regions, potentially causing aggregation
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