For GSSG measurement, 2-vinylpyridine (Sigma-Aldrich) was added before the enzymatic reactions to remove reduced glutathione
F.MaurerE.BushA.NielsenK
Melanin production is known to decrease and become less efficient with aging in melanocytes, with fewer melanosomes produced and poor quality/quantity of melanin 8,71
1 H NMR (300 MHz, DMSO) 8.26 (d, J = 7.6 Hz, 1H), 7.45 7.34 (m, 9H), 7.25 (d, J = 1.4 Hz, 1H), 7.13 7.03 (m, 6H), 7.03 6.95 (m, 1H), 6.70 6.61 (m, 1H), 4.49 (q, J = 6.9 Hz, 1H), 3.54 (s, 3H), 3.52 (d, J = 5.9 Hz, 1H), 2.82 (dd, J = 6.7, 3.4 Hz, 2H), 1.35 (s, 9H)
The entire process takes just a few minutes, making it convenient for even the busiest schedules
While GHK-Cu is a well-researched topical ingredient, injectable GHK-Cu peptides are neither FDA approved nor regulated and aren't recommended