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Gene expression levels were calculated using the 2 -Ct method [ and expressed as relative units (RU) compared to the untreated controls where the level of gene expression was considered to be equal to 1

Over time, follicles shrink, thin out, or go dormant entirely

10.1111/j.1399-3054.2004.00424.x 125 PieterseC

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If insulin were injected directly into muscle, which has robust blood supply, it would be absorbed much more rapidly, producing a sudden spike in circulating insulin that could cause dangerous hypoglycemia