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In vivo , DK-3 treatment reduced tau phosphorylation (Thr181, Thr205, Ser396) and inflammation markers such as IL-6, CoX-2, and IL-1, alleviating neuronal damage (An et al., 2021)

Briefly, RAW 264.7 cells were washed three times with phosphate buffer saline (PBS, Corning) and then incubated in ice-cold hypotonic lysing buffer (50 mM TrisHCl, 10 mM magnesium sulfate at pH 7.5) for 4 h at 4 C

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High cell density cultivation process for the expression of botulinum neurotoxin a receptor binding domain