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PMID: 26500843

On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

Abstract This chapter describes the use of glutathione S-transferase (GST) gene fusion proteins as a method for inducible, high-level protein expression and purification from bacterial cell lysates

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Structurally, BPC-157 is small enough to be synthesized at scale, stable enough at gastric pH to survive oral administration in some experimental conditions, and short enough that the immune system rarely treats it as foreign