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In vivo animal experiments showed that daily administration of NNMTi for 28 days to dietinduced obese (DIO) mice significantly upregulated the expression of lipolysisrelated genes (such as ATGL and HSL) and downregulated lipogenesisrelated genes (such as FAS and ACC), indicating that NNMTi effectively promotes fat breakdown and inhibits fat synthesis

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Pulmonary administration both permits direct drug delivery to the lungs for respiratory applications and offers a non-invasive alternative to intravenous administration for systemic drug delivery 98,99,100

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