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The natural active GLP-1(737), hereafter referred to simply as GLP-1 in this article, is rapidly degraded by dipeptidyl peptidase 4 (DPP-4), which cleaves between residues Ala8 and Glu9, and is swiftly cleared renally within 12 min

In contrast, the exendin-P5 mutagenesis profile was very distinct from the other peptides with few mutations altering exendin-P5 affinity and only a very weak, albeit significant, correlation with the effect of mutations on GLP-1 in cAMP signalling assays (Figs

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